FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Myo31DF, arm
FlyBase ID
FBig0000095763
Interaction Network
Interactions Browser links
Myo31DF network
arm network
Reported Interactions
FBrf0191256-1.B2H
Reference
Speder et al., 2006
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Myo31DF 

Myo31DF

bait

fused to bacteriophage lambda repressor protein DNA-binding domain

Myo31DF 
arm 

Arm

prey

fused to alpha subunit of RNA polymerase

arm 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
arm 
arm repeats 6-12
sufficient binding region
aa 349-721

coordinates relative to arm-PA

Myo31DF 
tail domain
sufficient binding region
aa 737-1011
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was E. coli cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Speder et al., 2006)

Two-hybrid system: Bacteriomatch II system (Stratagene)

(Speder et al., 2006)
FBrf0191256-2.PD.A
Reference
Speder et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
arm 

Arm

bait

GST tag

arm 
Myo31DF 

Myo31DF

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
arm 
C-terminal region
sufficient binding region
aa 154-843
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Speder et al., 2006)
FBrf0191256-3.PD.WB
Reference
Speder et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
arm 

Arm

bait

GST tag

arm 
Myo31DF 

Myo31DF

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
arm 
C-terminal region
sufficient binding region
aa 154-843
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

(Speder et al., 2006)

S2 cells were transfected with a plasmid containing UAS-Myo31DF driven by another transfected plasmid containing actin-GAL4. The subsequent Western blot was probed with an anti-Myo31DF antibody.

(Speder et al., 2006)
FBrf0218056-1.coIP.WB
Reference
Petzoldt et al., 2012
Description
physical association
Assay
Collection
Source/Stage
/ third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Myo31DF 

MyoID

bait

GFP tag

arm 

β-Catenin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The interaction of Myo31DF (MyoIC) and arm is disrupted by overexpression of Myo61F (MyoID).

(Petzoldt et al., 2012)

Bait derived from transgenic line carrying GFP-tagged Myo31DF construct, P{UAS-Myo31DF.GFP}.

(Petzoldt et al., 2012)

Prey endogenous to larval extract.

(Petzoldt et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)