Bait protein was fused to the motor domain of kinesin heavy chain (Khc), which targets the chimera to the plus end of microtubules. The ability of this chimera to recruit prey protein to the plus end of microtubules was taken as evidence of direct physical interaction.
Source was larval wing discs of transgenic fly line; bait produced from tagged transgenic construct; prey produced from transgenic construct.
Source was S2 cell line transfected with tagged cos constructs.
Separate molecules tagged with CFP and YFP; assaying homodimerization.
Source was wing discs from transgenic line carrying tagged cos constructs.
Separate molecules tagged with CFP and YFP; assaying homodimerization.
Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.
