FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
fu, smo
FlyBase ID
FBig0000095786
Interaction Network
Interactions Browser links
fu network
smo network
Reported Interactions
FBrf0167606-2.coIP.WB
Reference
Jia et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait

FLAG tag

smo 
fu 

Fu

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
smo 
C-terminal region
sufficient binding region
aa 730-1035
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Jia et al., 2003)
FBrf0167606-4.coIP.WB
Reference
Jia et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait

Myc tag

smo 
fu 

Fu

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
smo 
C-terminal region
necessary binding region
aa 556-1035
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Jia et al., 2003)
FBrf0167606-5.coIP.WB
Reference
Jia et al., 2003
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait

Myc tag

smo 
fu 

Fu

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
smo 
C-terminal region
necessary binding region
aa 556-1035
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval wing disc of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Jia et al., 2003)
FBrf0167876-12.coIP.MS
Reference
Lum et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
CME-W1-Cl.8+
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait
fu 

Fu

prey

2 unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of clone8 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lum et al., 2003)
FBrf0167876-4.coIP.WB
Reference
Lum et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait
fu 

Fu

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lum et al., 2003)
FBrf0167937-2.coIP.WB
Reference
Ruel et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
CME-W1-Cl.8+
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait
fu 

Fu

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of clone8 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Ruel et al., 2003)
FBrf0179063-10.coIP.WB
Reference
Ogden et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait
fu 

Fu

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Ogden et al., 2003)
FBrf0179063-2.coIP.WB
Reference
Ogden et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait
fu 

Fu

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Ogden et al., 2003)
FBrf0179063-6.coIP.WB
Reference
Ogden et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait
fu 

Fu

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Ogden et al., 2003)

The interaction of smo and cos is not affected by the expression of hh in S2 cells.

(Ogden et al., 2003)
FBrf0194316-1.Y2H
Reference
Malpel et al., 2007
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
fu 

FU

bait
fu 
smo 

SMO

prey

15 independent smo cDNAs recovered

smo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
fu 
region
sufficient binding region
aa 306-805

\'FU REG\'

smo 
C terminus
sufficient binding region
aa 985-1036

\'SMO cytotail\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Prey derived from embryonic cDNA library.

(Malpel et al., 2007)

Two-hybrid system: yeast GAL4-BD/GAL4-AD.

(Malpel et al., 2007)
FBrf0217531-3.coIP.WB
Reference
Ranieri et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
CME-W1-Cl.8+
Participants
Corresponds to
Reported as
Role
Note
smo 

smo

bait
fu 

fu

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of CME-W1-Cl.8+ cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Ranieri et al., 2012)

Cells treated with hh.

(Ranieri et al., 2012)
FBrf0217531-7.coIP.WB
Reference
Ranieri et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
CME-W1-Cl.8+
Participants
Corresponds to
Reported as
Role
Note
fu 

fu

bait
smo 

smo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of CME-W1-Cl.8+ cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Ranieri et al., 2012)

Cells treated with hh.

(Ranieri et al., 2012)
FBrf0226417-5.coIP.WB
Reference
Ranieri et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
CME-W1-Cl.8+
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait
fu 

Fu

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of CME-W1-Cl.8+ cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Ranieri et al., 2014)
FBrf0226417-8.coIP.WB
Reference
Ranieri et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
fu 

Fu

bait

FLAG tag

smo 

Smo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Ranieri et al., 2014)
FBrf0240921-1.coIP.WB
Reference
Giordano et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
fu 

Fu

bait

Myc or HA tag

fu 
smo 

Smo

prey

HA or Myc tag

smo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
smo 
interaction domain
sufficient binding region
aa 985-1036
fu 
regulatory domain
sufficient binding region
aa 303-805
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The interaction between fu and the C-terminal 52 aa of smo is enhanced in the presence of full length smo and hh.

(Giordano et al., 2018)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Giordano et al., 2018)
FBrf0240921-2.BiFC.FM
Reference
Giordano et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait

YFP tag, N-terminal fragment, Myc tag

fu 

Fu

prey

YFP tag, C-terminal fragment, HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The interaction between fu and smo is increased in the presence of hh, Pka-C1 and Gprk2 and decreased in the presence of ptc.

(Giordano et al., 2018)

Source was live S2 cells; proteins produced from transfected constructs.

(Giordano et al., 2018)
FBrf0240921-3.coIP.WB
Reference
Giordano et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait

HA tag

smo 
fu 

Fu

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
smo 
interaction domain
mutation decreasing interaction
aa 985-1036
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Giordano et al., 2018)
FBrf0240921-4.BiFC.FM
Reference
Giordano et al., 2018
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait

YFP tag, N-terminal fragment, Myc tag

fu 

Fu

prey

YFP tag, C-terminal fragment, HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was wing discs from transgenic fly line; proteins produced from transgenic constructs.

(Giordano et al., 2018)
FBrf0257154-16.CL.FM
Reference
Kubíková et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait

EGFP used to maintain membrane localization

fu 

Fused

prey

mCherry tag

fu 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
fu 
CTD residues 495-805
necessary binding region
aa 495-805

coordinates relative to fu-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2R+ cells; bait and prey produced from transfected construct.

(Kubíková et al., 2023)

Bait protein was fused to plasma membrane anchor. The ability of this membrane bound bait to recruit prey protein to the plasma membrane was taken as evidence of direct physical interaction.

(Kubíková et al., 2023)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (9)