FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
numb, AP-2α
FlyBase ID
FBig0000095791
Interaction Network
Interactions Browser links
numb network
AP-2α network
Reported Interactions
FBrf0151895-1.PD.A
Reference
Berdnik et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

nb

bait

GST tag

numb 
AP-2α 

Ada

prey

[35]S label

AP-2α 
AP-2α 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
AP-2α 
ear domain
mutation disrupting interaction
aa 824-825

ΔV824-N825

AP-2α 
ear domain
necessary binding region
aa 841-940
numb 
α-Adaptin binding DPF motif
necessary binding region
aa 426-546

deletion of this region weakens interaction

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Berdnik et al., 2002)
FBrf0151895-2.coIP.WB
Reference
Berdnik et al., 2002
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

Nb

bait
AP-2α 

Ada

prey
AP-2α 
AP-2α 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
AP-2α 
ear domain
mutation disrupting interaction
aa 824-825

ΔV824-N825

AP-2α 
ear domain
necessary binding region
aa 841-940
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Berdnik et al., 2002)
FBrf0187299-2.coIP.WB
Reference
Bhalerao et al., 2005
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

Numb

bait

MYC tag

AP-2α 

α-Ada

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Bhalerao et al., 2005)

A mutant form of numb, numb S52F, bound similar amounts of AP-2α as compared to wild type numb

(Bhalerao et al., 2005)
FBrf0206088-18.PD.WB
Reference
Wirtz-Peitz et al., 2008
Description
physical association
Assay
Collection
Source/Stage
larval brain / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

Numb

bait

GST tag

AP-2α 

α-adaptin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

numb pulls down baz, par-6, AP-2α and aPKC, but not l(2)gl.

(Wirtz-Peitz et al., 2008)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type larval brain extract.

(Wirtz-Peitz et al., 2008)
FBrf0206088-21.PD.WB
Reference
Wirtz-Peitz et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

Numb

bait

GST tag

numb 
AP-2α 

α-adaptin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
numb 
region
mutation disrupting interaction
aa 52

S52F

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryo extract.

(Wirtz-Peitz et al., 2008)

numb pulls down baz, par-6, AP-2α and aPKC, but not l(2)gl.

(Wirtz-Peitz et al., 2008)
FBrf0218342-3.coIP.WB
Reference
Song and Lu, 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AP-2α 

Ada

bait

FLAG tag

AP-2α 
numb 

Numb

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
AP-2α 
Trunk
sufficient binding region
aa 1-621
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in transfected HEK-293T cells.

(Song and Lu, 2012)

Prey expressed in transfected HEK-293T cells.

(Song and Lu, 2012)
FBrf0221275-3.coIP.WB
Reference
Cotton et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
numb 

Nb

bait

Myc tag

AP-2α 

AP-2α

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Cotton et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)