Cul3[[Testis]]
fused to GAL4 DNA-binding domain
Klhl10
fused to GAL4 activation domain
coordinates relative to Cul-3-PF
Two-hybrid system: yeast GAL4-BD/GAL4-AD.
The testis-specific Cul-3 N-terminus is necessary but not sufficient for strong interaction with klhl10.
Bait was previously cloned EST (AT19493).
Prey derived from an adult Drosophila cDNA library.
Cul3T
FLAG tag
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
