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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000095938
Interaction Network
Interactions Browser links
Cul3 network
klhl10 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0202727-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cul3 

Cul3[[Testis]]

bait

fused to GAL4 DNA-binding domain

Cul3 
Cul3 

Klhl10

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cul3 
testis-specific N-terminus
necessary binding region
aa 1-181

coordinates relative to Cul-3-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD.

The testis-specific Cul-3 N-terminus is necessary but not sufficient for strong interaction with klhl10.

Bait was previously cloned EST (AT19493).

Prey derived from an adult Drosophila cDNA library.

FBrf0231856-5.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cul3 

Cul3T

bait

FLAG tag

Klhl10

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)