FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Rok, Rho1
FlyBase ID
FBig0000095948
Interaction Network
Interactions Browser links
Rok network
Rho1 network
Reported Interactions
FBrf0111979-1.Y2H
Reference
Mizuno et al., 1999
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rok 

DRho-kinase

bait

fused to GAL4 activation domain

Rok 
Rok 
Rho1 

Drho1

prey

fused to LexA DNA-binding domain

Rho1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rok 
conserved Rho-binding site
unspecified role
aa 912-1107
Rok 
region
unspecified role
aa 1017-1018

necessary: mutation abolishes interaction

Rho1 
region
unspecified role
aa 1-192

sufficient, lacks C-terminal CLLL membrane targeting motif

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

In this assay, rok protein interacts specifically with a mutant Rho1 sequence that mimics the active, GTP-bound state (G14V); there is no interaction with wildtype or a mutant that mimics the inactive GDP-bound state of Rho1 (T19N).

(Mizuno et al., 1999)

Two-hybrid system: yeast LexA/GAL4-AD.

(Mizuno et al., 1999)
FBrf0135688-1.PD.A
Reference
Winter et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rho1 

RhoA

bait

GST tag

Rho1 
Rok 

DROK

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rho1 
effector-binding domain
mutation disrupting interaction
aa 37

T37A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The V14 activated form of Rho1 was pre-loaded with GTP and tested for binding to rok.

(Winter et al., 2001)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Winter et al., 2001)

rok binds Rho1 but not other GTPases Rac1 or Cdc42.

(Winter et al., 2001)
FBrf0194289-1.PD.WB
Reference
Nahm et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rok 

Rok

bait

GST tag

Rok 
Rho1 

RhoA

prey

MYC tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rok 
p21 binding domain
sufficient binding region
aa 960-1054
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Nahm et al., 2006)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)