FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Rab7, Vps35
FlyBase ID
FBig0000096857
Interaction Network
Interactions Browser links
Rab7 network
Vps35 network
Reported Interactions
FBrf0220559-1.PD.WB
Reference
Dong et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Rab7 

Rab7

bait

GST tag

Vps35 

Vps35

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced from transfected construct in S2 cells.

(Dong et al., 2013)

Vps25 binds both GDP- and GTP-loaded forms of Rab7.

(Dong et al., 2013)
FBrf0254347-1.coIP.WB
Reference
Asadzadeh et al., 2022
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Vps35 

Vps35

bait

HA tag

Rab7 

Rab7

prey

GFP tag

Rab7 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rab7 
GDP locked residue 22
mutation disrupting interaction
aa 22

T22N; coordinates relative to Rab7-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Asadzadeh et al., 2022)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)