FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
kel, Cul3
FlyBase ID
FBig0000096874
Interaction Network
Interactions Browser links
kel network
Cul3 network
Reported Interactions
FBrf0209686-1.coIP.WB
Reference
Hudson and Cooley, 2010
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cul3 

CUL-3

bait

FLAG tag

kel 

Kel1B

prey

SBP, mCherry tags

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Tagged Cul-3 interacts with tagged and endogenous kel.

(Hudson and Cooley, 2010)

Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Hudson and Cooley, 2010)
FBrf0209686-2.PD.WB
Reference
Hudson and Cooley, 2010
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
kel 

Kel1B

bait

SBP, mCherry tags

Cul3 

CUL-3

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Streptavidin-based purification of kel complexes via SBP tag.

(Hudson and Cooley, 2010)

Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Hudson and Cooley, 2010)
FBrf0221234-2.coIP.WB
Reference
Strutt et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
kel 

Kelch

bait

Myc tag

Cul3 

Cul3

prey

Venus tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Strutt et al., 2013)
FBrf0230137-1.coIP.WB
Reference
Hudson et al., 2015
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
kel 

Kelch

bait

SBP tag

Cul3 

Cul3

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult ovaries of transgenic fly line; bait produced from transgenic gene; prey produced from transgenic gene.

(Hudson et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)