Interaction of GEFmeso with the GTPase Cdc42 was tested in the absence of nucleotides or in the presence of non-hydrolyzable GTPγS.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

GEFmeso interacts with Cdc42 only in the absence of nucleotides. The interaction is inhibited in the presence of non-hydrolyzable GTPγS.

GEFmeso interacts specifically with Cdc42, and not other Drosophila Rho GTPases (Rho1, Rac1, Mtl, RhoBTB or RhoL).