DmGfrl
V5 tag
FasIIa
HA or FLAG tag
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Gfrl interacts with Fas2 but not Sdc.
N-terminally HA-tagged Fas2 was more efficiently coimmunoprecipitated with Gfrl than C-terminally FLAG-tagged Fas2.
Antibody for immunoprecipitation targeted Gfrl polypeptide, not the V5 tag.