Interactions were reported only if the following criteria were met; protein was not found in negative control samples (cells transfected with empty vector or Mef2-expressing vector); protein was found in more than one replicate, with a Mascot score > 50; protein was not identified by just a single small peptide (<10 residues).

CG6904, MAPk-Ak2, CG6509, Cdc16, PTP-ER, ush and CG8108 are likely to be p38 kinase substrates, given that observed phosphosites in these proteins overlap predicted p38 kinase target motifs.

Source was extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.