Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Interaction in vitro; proteins produced as a recombinant fusion proteins in baculovirus and insect cell system.
Hexameric CCR4-NOT-mini complex captured as 15 pairwise interactions.
Source was live S2R+ cells; bait and prey produced from endogenous genes.
Not3 was fused to a plasma membrane anchor. The ability of this chimera to recruit prey protein to the plasma membrane was taken as evidence of direct physical interaction.
