Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transgenic embryonic extract.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Positive control.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Positive control.
Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced by in vitro translation.
Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from wild-type embryo extract.
Stoichiometry approximately 1:1 regardless of RNA length.
Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from wild-type embryo extract.
Stoichiometry approximately 1:1 regardless of RNA length.
Two-hybrid system: yeast GAL4-BD/GAL4-AD; positive control
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Source was live S2R+ cells; bait and prey produced from endogenous genes.
cup was fused to a plasma membrane anchor. The ability of this chimera to recruit prey protein to the plasma membrane was taken as evidence of direct physical interaction.
