S2 cells were treated with cycloheximide to induce apoptosis, and the lysate was tested for binding to drpr.

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-Sf21 cell system; prey derived from apoptotic S2 cell extract.

S2 cells were treated with cycloheximide to induce apoptosis, and the lysate was tested for binding to drpr.

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-Sf21 cell system; prey derived from apoptotic S2 cell extract.

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-Sf9 cell system; prey produced as a recombinant fusion protein in bacterial system.

Any single EGF repeat of the drpr extracellular domain seems to be sufficient for binding to prtp (only three of 15 EGF repeats were directly tested).