Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast LexA-BD/B42-AD

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Cells were exposed to light for 15 minutes before harvesting.

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus and insect cell system; prey produced as a recombinant fusion protein in baculovirus and insect cell system.

The jet-cry interaction is much stronger when the reaction mixture is exposed to blue light. The light enhanced affinity of cry for jet decays with a half-life of 15 min.

The reaction mixtures were incubated in the dark or under light for 12.5 minutes.

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected Sf21 cell system; prey produced as a recombinant fusion protein in baculovirus-infected Sf21 cell system.

The cry-jet interaction is enhanced by light.

cry binds to jet more strongly than to BRWD3.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in Sf21 insect cell system.

Mutations to various cry tryptophan residues (W397F, W536F) do not perturb the cry-jet interaction.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Positive control.