FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Sas-4, Plp
FlyBase ID
FBig0000097172
Interaction Network
Interactions Browser links
Sas-4 network
Plp network
Reported Interactions
FBrf0216370-10.coIP.WB
Reference
Gopalakrishnan et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 12blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9embryonic stage 5embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sas-4 

Sas-4

bait

TAP tag

Plp 

D-PLP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Gopalakrishnan et al., 2011)

Characterization of Sas-4 complexes shows that Sas-4 and cp309 cosediment in a narrow range of fractions at 7S.

(Gopalakrishnan et al., 2011)

Coimmunoprecipitation from "high-speed lysates" depleted of mature centrosomes.

(Gopalakrishnan et al., 2011)
FBrf0216370-36.PD.WB
Reference
Gopalakrishnan et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 12blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9embryonic stage 5embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sas-4 

Sas-4

bait

GST tag

Sas-4 
Sas-4 
Plp 

D-PLP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sas-4 
tubulin binding domain
sufficient binding region
aa 1-190
Sas-4 
PN2-3 domain
necessary binding region
aa 103-121
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Gopalakrishnan et al., 2011)
FBrf0216370-4.coIP.MS
Reference
Gopalakrishnan et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 12blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9embryonic stage 5embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sas-4 

Sas-4

bait
Plp 

D-PLP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gopalakrishnan et al., 2011)

Coimmunoprecipitation from "high-speed lysates" depleted of mature centrosomes.

(Gopalakrishnan et al., 2011)
FBrf0219801-11.coIP.WB
Reference
Gopalakrishnan et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sas-4 

Sas-4

bait
Plp 

D-PLP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gopalakrishnan et al., 2012)

cp309 coimmunoprecipitates and co-fractionates with Sas-4 as part of the S-CAP complex (centrosomal scaffold).

(Gopalakrishnan et al., 2012)

Sas-4 complexes were immunoprecipitated from "high-speed lysates" depleted of mature centrosomes and different Sas-4-containing complexes were fractionated on a sucrose gradient.

(Gopalakrishnan et al., 2012)
FBrf0219801-50.coIP.WB
Reference
Gopalakrishnan et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sas-4 

Sas-4

bait
Plp 

D-PLP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gopalakrishnan et al., 2012)

The interaction of Sas-4 with cp309 is disrupted by the addition of a non-hydrolyzable GTP analog, GMPCPP, to the extract.

(Gopalakrishnan et al., 2012)

Coimmunoprecipitation from "high-speed lysates" depleted of mature centrosomes.

(Gopalakrishnan et al., 2012)
FBrf0233282-122.Y2H
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Plp 

PLP

bait

fused to GAL4 DNA-binding domain

Plp 
Sas-4 

Sas-4

prey

fused to GAL4 activation domain

Sas-4 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Plp 
region F3
sufficient binding region
aa 1377-1811

coordinates relative to Plp-PF

Sas-4 
N-terminal region
sufficient binding region
aa 1-347

coordinates relative to Sas-4-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Galletta et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Galletta et al., 2016)
FBrf0233282-123.Y2H
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sas-4 

Sas-4

bait

fused to GAL4 DNA-binding domain

Sas-4 
Plp 

PLP

prey

fused to GAL4 activation domain

Plp 
Plp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Plp 
region F2
sufficient binding region
aa 584-1376

coordinates relative to Plp-PF

Plp 
region F3
sufficient binding region
aa 1377-1811

coordinates relative to Plp-PF

Sas-4 
N-terminal region
sufficient binding region
aa 1-347

coordinates relative to Sas-4-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Galletta et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Galletta et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)