Source was cell extract of SL2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

msl-1, msl-2 and msl-3 also copurify in various chromatographic fractionation studies.

Source was cell extract of SL2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

msl-1, msl-2 and msl-3 also copurify in various chromatographic fractionation studies.

Positive control.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

The msl-3-msl-2 interaction is detected only in the presence of msl-1.

Source was cell extract of baculovirus-infected Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.

Source was cross-linked nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Crude nuclear extracts were cross-linked using formaldehyde, sonicated, and subjected to ProteinA affinity purification with rabbit IgG agarose beads eluted under denaturing conditions, and subsequently using streptavidin agarose beads for Bio affinity purification. Purified peptides were eluted by on-bead trypsin digestion and mass spectrometric analysis.

Enrichment of prey protein was compared to input, mock and unrelated BioTAP affinity purifications using a statistical method (Bamse) developed by the authors to control for multiple sources of bias.

Binding of msl-2 to msl-3 is RNA-independent (resistant to RNase treatment).

Positive control.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Factors were detected on polytene chromosomes using antibodies (to proteins) or biotinylated antisense probes (to ncRNA). The PLA signal develops only if probes to the two factors are in close proximity to each other (within tens of nanometers).

Source was larval salivary gland of wild-type fly line; proteins produced from endogenous gene.

Interaction in vitro; bait and prey produced as a recombinant fusion protein in baculovirus and insect cell system.

FLAG tagged msl-2 was used to purify the 4-MSL complex (msl-2, msl-1, msl-3 and mof). Captured as 3 pairwise interactions utilizing msl-2 as bait.