Two-hybrid system: yeast GAL4-BD/GAL4-AD

This interaction was not observed in the reciprocal experiment (where bait and prey roles were switched).

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

Tfb5 outcompetes CG31917 for binding to mrn.

mrn coimmunoprecipitates with each Tfb5 and CG31917, but Tfb5 and CG31917 do not interact with each other in this assay.

Tfb5 does not interact with other TFIIH components Cdk7 or hay in this assay.

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.