polybromo can immunoprecipitate brm and mor, but not osa, suggesting that osa and polybromo exist in distinct Brahma complexes.

Source was embryonic nuclear extract wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

brm, mor, dalao, Bap60, Bap55, Snr1 and actin form the Brahma (BRM) complex, together with either osa (BAP BRM complex) or polybromo and Bap170 (PBAP BRM complex).

Source was embryonic nuclear extract wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Nuclear extract was fractionated by ammonium sulfate precipitation and several chromatographic steps before being subjected to immunoprecipitation.

polybromo is present with brm, mor and Bap170 in a distinct Brahma complex that lacks osa.

Source was embryonic nuclear extract wild-type fly line; proteins produced from endogenous gene.

Nuclear extract was subjected to several chromatographic steps to isolate brm-containing complexes.

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

brm interacts with polybromo and osa, but osa and polybromo do not interact, suggesting two distinct brm-containing complexes.