Positive control.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

This interaction persists upon RNase treatment.

The interaction of msl-2 with mof is not disrupted by high salt.

Source was cell extract of SL2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

mof was precipitated at higher levels from cells stably transfected with an msl-2-GFP plasmic. However, some mof was precipitated from wild type cells by an antibody to endogenous msl-2.

Source was cell extract of Sf4 derived cell line; bait produced from stably transfected construct or endogenous gene; prey produced from endogenous gene.

Positive control.

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

Factors were detected on polytene chromosomes using antibodies (to proteins) or biotinylated antisense probes (to ncRNA). The PLA signal develops only if probes to the two factors are in close proximity to each other (within tens of nanometers).

Source was larval salivary gland of wild-type fly line; proteins produced from endogenous gene.

FLAG tagged msl-2 was used to purify the 4-MSL complex (msl-2, msl-1, msl-3 and mof). Captured as 3 pairwise interactions utilizing msl-2 as bait.

Interaction in vitro; bait and prey produced as a recombinant fusion protein in baculovirus and insect cell system.