FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
CtBP
FlyBase ID
FBig0000097496
Interaction Network
Interactions Browser links
CtBP network
Reported Interactions
FBrf0102176-4.Y2H
Reference
Poortinga et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CtBP 

CtBP

bait

fused to LexA DNA-binding domain

CtBP 

CtBP

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Poortinga et al., 1998)

Two-hybrid system: yeast LexA-BD/VP16-AD

(Poortinga et al., 1998)
FBrf0213805-1.coIP.WB
Reference
Bhambhani et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
CtBP 

CtBP

bait

FLAG tag

CtBP 

CtBP

prey

HA or V5 tag

CtBP 
CtBP 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CtBP 
dimerization domain residues 134,138,141,142
mutation disrupting interaction
aa 134,138,141,142

C134Y,N138R,R141A,R142A

CtBP 
dimerization domain residues 171,173
mutation disrupting interaction
aa 171,173

R171E,R173E. Co-expression of complementary salt-bridge mutant E126R,E127R restores dimerization.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bhambhani et al., 2011)
FBrf0237493-1.coIP.WB
Reference
Bi et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
CtBP 

CtBP

bait

FLAG tag

CtBP 

CtBP

prey

V5 tag

CtBP 
CtBP 
CtBP 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CtBP 
dimerization domain residues 141,142
mutation disrupting interaction
aa 141,142

R141A,R142A, coordinates relative to CtBP-PF

CtBP 
dimerization domain residue 171
mutation disrupting interaction
aa 171

R171A, coordinates relative to CtBP-PF

CtBP 
NAD binding domain residue 183
mutation disrupting interaction
aa 183

G183V, coordinates relative to CtBP-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bi et al., 2018)

Addition of NADH enhanced CtBP dimerization.

(Bi et al., 2018)
External Crossreferences and Linkouts ( 0 )
References (3)