FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Not11, Not1
FlyBase ID
FBig0000097543
Interaction Network
Interactions Browser links
Not11 network
Not1 network
Reported Interactions
FBrf0221179-24.coIP.WB
Reference
Bawankar et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Not11 

NOT11

bait

GFP tag

Not1 

NOT1

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bawankar et al., 2013)

The interaction of Not1 with Not11 is much stronger in the presence of Not10.

(Bawankar et al., 2013)
FBrf0221179-26.PD.MW
Reference
Bawankar et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Not1 

NOT1

bait

GST tag

Not1 
Not11 

NOT11

prey

MBP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Not1 
NOT10/11 binding domain
sufficient binding region
aa 1-412
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Bawankar et al., 2013)
FBrf0221179-27.PD.MW
Reference
Bawankar et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Not11 

NOT11

bait

MBP tag

Not1 

NOT1

prey

GST tag

Not1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Not1 
NOT10/11 binding domain
sufficient binding region
aa 1-412
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Bawankar et al., 2013)
FBrf0254621-5.coIP.WB
Reference
Haugen et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Not1 

Not1

bait

Flag tag

Not11 

Not11

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control

(Haugen et al., 2022)

Source was cell extract of DL1 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Haugen et al., 2022)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)