Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Cholesterol modification of hh is required for its homodimerization.
Wild-type and the C85S, non-palmitoylated, form of hh formed multimers, while a truncated version consisting of aa 1-257 did not.
Source was larvae of transgenic fly line; protein produced from transgenic construct.
The C85S, non-palmitoylated, form of hh formed multimers.
Source was medium of S2 cells; protein produced from transfected construct.
C-terminal tags impair hh multimerization.
Source was larvae of transgenic fly line; protein produced from transgenic construct.
