Source was larvae of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Coimmunoprecipitation done using subcellular fractions enriched for plasma membrane and transport vesicles.

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

hh is bound and endocytosed by cells expression ptc; ptc and hh colocalize that the cell surface and in endocytic vesicles.

Cells expressing ptc were mixed with conditioned medium containing secreted hh protein.

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus system; prey produced as a recombinant fusion protein in unspecified cell line.

High affinity binding of hh requires both ptc and ihog.

Cell-free binding of hh to vesicles containing ptc and/or ihog.

Interaction in vitro; bait produced as a recombinant fusion protein in unspecified cell line; prey produced as a recombinant fusion protein in S2R+ cell line.

The binding of solubilized ptc to hh requires coexpression of ihog or boi.

hh was bound to beads then mixed with detergent-solubilized extracts of transfected S2R+ cell line.