FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
dock, Ack
FlyBase ID
FBig0000097602
Interaction Network
Interactions Browser links
dock network
Ack network
Reported Interactions
FBrf0147103-1.PD.ED
Reference
Worby et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
dock 

Dock

bait

His tag

dock 
Ack 

p145

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dock 
SH2 domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait was affinity purified by virtue of the His tag, and proteins were further purified by an anti-phosphotyrosine resin.

(Worby et al., 2002)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Worby et al., 2002)
FBrf0147103-4.coIP.WB
Reference
Worby et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
dock 

Dock

bait
Ack 

DAck

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Worby et al., 2002)
FBrf0147103-5.PD.WB
Reference
Worby et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
dock 

Dock

bait

GST tag

dock 
Ack 

DAck

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dock 
SH2 domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

(Worby et al., 2002)
FBrf0147103-8.Y2H
Reference
Worby et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dock 

Dock

bait

fused to LexA DNA-binding domain

Ack 

DAck

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Worby et al., 2002)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Worby et al., 2002)
FBrf0221591-1.coIP.WB
Reference
Abdallah et al., 2013
Description
physical association
Assay
Collection
Source/Stage
testis / adult stagemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dock 

dock

bait
Ack 

Ack

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult testis of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Abdallah et al., 2013)
FBrf0221591-2.coIP.WB
Reference
Abdallah et al., 2013
Description
physical association
Assay
Collection
Source/Stage
testis / adult stagemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dock 

dock

bait
Ack 

Ack

prey

mCherry tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult testis of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

(Abdallah et al., 2013)
FBrf0221591-3.coIP.WB
Reference
Abdallah et al., 2013
Description
physical association
Assay
Collection
Source/Stage
testis / adult stagemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dock 

dock

bait

GFP tag

dock 
Ack 

Ack

prey

mCherry tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dock 
SH2 domain
mutation decreasing interaction
aa 336

R336Q (coordinates relative to dock-PA isoform)

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult testis of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Abdallah et al., 2013)

Mutation of the dock SH2 domain strongly decreases the interaction with Ack.

(Abdallah et al., 2013)

Mutations in the SH3 domain of dock do not affect its interaction with Ack protein.

(Abdallah et al., 2013)

GFP-tagged dock protein detected using antibody to endogenous dock protein.

(Abdallah et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)