FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Roc1a, Ufd1
FlyBase ID
FBig0000097655
Interaction Network
Interactions Browser links
Roc1a network
Ufd1 network
Reported Interactions
FBrf0221943-10.coIP.WB
Reference
Zhang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Ufd1 

Ufd1-like

bait

T7 tag

Roc1a 

Roc1a

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhang et al., 2013)

When coexpressed, Roc1a, Ufd1-like and TER94 form a ternary complex.

(Zhang et al., 2013)

Two-step immunoprecipitation of FLAG-tagged Roc1a, then T7-tagged Ufd1-like (coexpression with V5-tagged TER94).

(Zhang et al., 2013)
FBrf0221943-5.PD.WB
Reference
Zhang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Roc1a 

Roc1a

bait

GST tag

Ufd1 

Ufd1

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

In vitro, Ufd1-like interacts specifically with Roc1a, but not skpA, lin19 or slmb.

(Zhang et al., 2013)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Zhang et al., 2013)
FBrf0221943-6.coIP.WB
Reference
Zhang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Roc1a 

Roc1a

bait

HA tag

Ufd1 

Ufd1-like

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhang et al., 2013)

Roc1a interacts with Ufd1-like, but not Npl4, in this assay.

(Zhang et al., 2013)
FBrf0221943-7.coIP.WB
Reference
Zhang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Roc1a 

Roc1a

bait

FLAG tag

Ufd1 

Ufd1-like

prey

T7 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhang et al., 2013)

When coexpressed, Roc1a, Ufd1-like and TER94 form a ternary complex.

(Zhang et al., 2013)

A direct interaction of His-tagged Ufd1-like with GST-tagged Roc1a was also observed in vitro.

(Zhang et al., 2013)

Two-step immunoprecipitation of FLAG-tagged Roc1a, then T7-tagged Ufd1-like (coexpression with V5-tagged TER94).

(Zhang et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)