FlyBase Gene Report: Dmel\Roc1a

General Information

Symbol

Dmel\Roc1a

Species

D. melanogaster

Name

Regulator of cullins 1a

Annotation Symbol

CG16982

Feature Type

protein_coding_gene

FlyBase ID

FBgn0025638

Gene Model Status

Current

Stock Availability

10 publicly available

Gene Summary

Regulator of cullins 1a (Roc1a) encodes a C2H2 type zinc finger protein and component of several Cullin-Ring E3 ubiquitin ligase complexes. It binds directly to the Cullin subunit within the CRL complex and functions to recruit ubiquitin-charged E2 proteins. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Gene Group
UniProtKB
Interactive Fly

Also Known As

Rbx1, dRbx1, EG:115C2.11 , Roc1

Key Links

Genomic Location

Cytogenetic map

1B13-1B13

Sequence location

X:647,304..648,597 [+]

Recombination map

1-0

RefSeq locus

NC_004354 REGION:647304..648597

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (22 terms)

Molecular Function (6 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

enables cullin family protein binding

inferred from physical interaction with FLYBASE:Cul1; FB:FBgn0015509

(Reynolds et al., 2008)

inferred from physical interaction with FLYBASE:Cul2; FB:FBgn0032956

(Reynolds et al., 2008)

inferred from physical interaction with FLYBASE:Cul4; FB:FBgn0033260

(Reynolds et al., 2008)

inferred from physical interaction with FLYBASE:Cul3; FB:FBgn0261268

(Reynolds et al., 2008)

enables protein binding

inferred from physical interaction with FLYBASE:Ufd1; FB:FBgn0036136

(Zhang et al., 2013)

enables ubiquitin protein ligase activity

inferred from direct assay

(Reynolds et al., 2008, Noureddine et al., 2002)

enables ubiquitin-protein transferase activity

inferred from genetic interaction with SGD:S000005493

(Bocca et al., 2001)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables cullin family protein binding

inferred from biological aspect of ancestor with PANTHER:PTN000129805

(GO Reference Genome Project, 2011-)

enables NEDD8 ligase activity

inferred from sequence or structural similarity with UniProtKB:P62877

(Gene Ontology Curators, 2002-)

enables ubiquitin protein ligase activity

inferred from biological aspect of ancestor with PANTHER:PTN000129805

(GO Reference Genome Project, 2011-)

enables zinc ion binding

non-traceable author statement

(Noureddine et al., 2002)

inferred from electronic annotation with InterPro:IPR024766

(InterPro Project Members, 2004-)

inferred from sequence model

(Ying et al., 2011)

Biological Process (11 terms)

Terms Based on Experimental Evidence (8 terms)

CV Term

Evidence

References

involved_in cell population proliferation

inferred from mutant phenotype

(Noureddine et al., 2002)

involved_in negative regulation of canonical Wnt signaling pathway

inferred from mutant phenotype

(Roberts et al., 2012)

involved_in negative regulation of insulin receptor signaling pathway

inferred from physical interaction with FLYBASE:Cul1; FB:FBgn0015509,FLYBASE:SkpA; FB:FBgn0025637,FLYBASE:slmb; FB:FBgn0283468

(Wong et al., 2013)

involved_in negative regulation of smoothened signaling pathway

inferred from mutant phenotype

(Donaldson et al., 2004, Noureddine et al., 2002)

involved_in positive regulation of neuron remodeling

inferred from mutant phenotype

(Wong et al., 2013)

involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process

inferred from mutant phenotype

(Roberts et al., 2012)

involved_in protein ubiquitination

inferred from direct assay

(Reynolds et al., 2008, Noureddine et al., 2002)

involved_in proteolysis

inferred from mutant phenotype

(Noureddine et al., 2002)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in protein neddylation

inferred from sequence or structural similarity with UniProtKB:P62877

(Gene Ontology Curators, 2002-)

involved_in protein ubiquitination

inferred from biological aspect of ancestor with PANTHER:PTN000129805

(GO Reference Genome Project, 2011-)

involved_in ubiquitin-dependent protein catabolic process

inferred from biological aspect of ancestor with PANTHER:PTN000129805

(GO Reference Genome Project, 2011-)

involved_in viral process

inferred by curator from GO:0031462

(Nayak et al., 2018)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

part_of Cul2-RING ubiquitin ligase complex

inferred from direct assay

(Reynolds et al., 2008, Donaldson et al., 2004)

inferred from physical interaction with FLYBASE:EloB; FB:FBgn0023212,FLYBASE:Cul2; FB:FBgn0032956,FLYBASE:EloC; FB:FBgn0266711

(Nayak et al., 2018)

part_of Cul3-RING ubiquitin ligase complex

inferred from direct assay

(Zhou et al., 2015, Reynolds et al., 2008)

part_of Cul4-RING E3 ubiquitin ligase complex

inferred from direct assay

(Reynolds et al., 2008)

located_in nucleus

inferred from direct assay

(Noureddine et al., 2002)

part_of SCF ubiquitin ligase complex

inferred from direct assay

(Wong et al., 2013, Zhang et al., 2013, Reynolds et al., 2008, Donaldson et al., 2004, Bocca et al., 2001)

inferred from mutant phenotype

(Roberts et al., 2012)

inferred from direct assay

(Noureddine et al., 2002)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000129805

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

ROC UBIQUITIN LIGASES

SCF-SLMB UBIQUITIN LIGASE COMPLEX

NEDD8 TRANSFERASES

VCB COMPLEX

Pathway (FlyBase)

NEGATIVE REGULATORS OF WNT-TCF SIGNALING PATHWAY

NEGATIVE REGULATORS OF INSULIN-LIKE RECEPTOR SIGNALING PATHWAY

NEGATIVE REGULATORS OF HEDGEHOG SIGNALING PATHWAY

Protein Family (UniProt)

Belongs to the RING-box family. (Q9W5E1)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

ROC UBIQUITIN LIGASES -: E3 (ubiquitin) ligases interact with ubiquitin-E2 (ubiquitin-conjugating) enzymes and the target protein, transferring the ubiquitin from the E2 to the substrate. Roc (Regulator of cullins)/RBX (Ring BoX) proteins are Ring Finger E3 ubiquitin ligases. They form the catalytic subunit of cullin-RING ubiquitin ligase complexes. (Adapted from FBrf0155667).
SCF-SLMB UBIQUITIN LIGASE COMPLEX -: SCF-slmb ubiquitin ligase complex ubiquitinates proteins, targeting them for degradation by the proteasome. (FBrf0217546).
NEDD8 TRANSFERASES -: NEDD8 transferases catalyse the transfer of the ubiquitin-like protein NEDD8 from one substrate to another. They include NEDD8 conjugating (E2) enzymes and NEDD8 ligase (E3) enzymes, which act sequentially in a process analogous to ubiquitination, and result in covalent attachment of NEDD8 to target proteins. Neddylation results in activation of members of the cullin-RING family of ubiquitin E3 ligases, which appear to be the major NEDD8 targets in the cell. (Adapted from PMID:25531226.)
VCB COMPLEX -: VCB complex (Vhl-EloC-EloB) is a member of a family of ubiquitin ligases that share a Cullin-Rbx module. VCB complex is a multiprotein complex, which consist of large modular assemblies comprising a RING domain, a scaffolding Cullin subunit, one or more adaptor proteins, and a substrate-recognition subunit. (Adapted from FBrf0129709, FBrf0240293 and PMID:15601820).

Pathway (FlyBase)

NEGATIVE REGULATORS OF WNT-TCF SIGNALING PATHWAY -: Negative regulators of Wnt-TCF (canonical Wnt) signaling down-regulate the pathway, resulting in the attenuation of transcriptional regulation mediated by β-catenin (arm).
NEGATIVE REGULATORS OF INSULIN-LIKE RECEPTOR SIGNALING PATHWAY -: Negative regulators of the Insulin-like Receptor signaling pathway suppress Insulin-like receptor (InR) activation or the activity of intracellular effectors. (Adapted from FBrf0232297, FBrf0230017 and FBrf0229989).
NEGATIVE REGULATORS OF HEDGEHOG SIGNALING PATHWAY -: Negative regulators of hedgehog signaling down-regulate the pathway, resulting in the repression of transcription of hh-responsive genes.

Protein Function (UniProtKB)

Core component of multiple SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Required for the specific SCF-dependent proteolysis of CI, but not that of ARM, suggesting that it also participates in the selection of substrates inside the SCF complex (PubMed:12062088). During early metamorphosis, part of the SCF-slmb complex that negatively regulates the InR/PI3K/TOR pathway to activate the pruning of unnecessary larval ddaC dendrites and mushroom body axons (PubMed:24068890). The SCF-slmb complex also regulates asymmetrical division of neuroblasts and inhibits ectopic neuroblast formation partly through SAK and Akt1 (PubMed:24413555). Also part of an SCF complex required for caspase activation during sperm differentiation (Probable).

(UniProt, Q9W5E1)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Roc1a for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9W5E1)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.39

Gene model reviewed during 5.52

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Roc1a-RA

FBtr0070122

NM_130496

696

108

Roc1a-RC

FBtr0308651

NM_001144671

1229

136

Roc1a-RD

FBtr0343564

NM_001297825

1145

108

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Roc1a-PA

FBpp0070117

12.5

108

5.98

FBpp0300874

15.7

136

6.97

FBpp0310165

12.5

108

5.98

Q9W5E1

NP_001284754

AHN59225

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

108 aa isoforms: Roc1a-PA, Roc1a-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Component of SCF E3 ubiquitin-protein ligase complexes consisting of Skpa, Cul1, Roc1a and an F-box protein. In larvae neuroblast self renewal and asymmetric division, as well as ddaC dendrite and mushroom body axon pruning, the complex contains the F-box protein slmb (SCF-slmb) (PubMed:24068890, PubMed:24413555). Interacts directly with Cul1 and Slmb (PubMed:11500045). In caspase activation during sperm differentiation, the complex contains the F-box protein ntc (Probable).

(UniProt, Q9W5E1)

Domain

The RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion (By similarity).

(UniProt, Q9W5E1)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Roc1a using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Roc1a_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.99

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

organism

(Noureddine et al., 2002)

larval stage

wing disc | ubiquitous

(Noureddine et al., 2002)

Additional Descriptive Data

Transcript is detected throughout development by RT-PCR. The transcript is expressed uniformly throughout the wing imaginal disc.

(Noureddine et al., 2002)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of Cul2-RING ubiquitin ligase complex

inferred from direct assay

(Reynolds et al., 2008, Donaldson et al., 2004)

inferred from physical interaction with FLYBASE:EloB; FB:FBgn0023212,FLYBASE:Cul2; FB:FBgn0032956,FLYBASE:EloC; FB:FBgn0266711

(Nayak et al., 2018)

part_of Cul3-RING ubiquitin ligase complex

inferred from direct assay

(Zhou et al., 2015, Reynolds et al., 2008)

part_of Cul4-RING E3 ubiquitin ligase complex

inferred from direct assay

(Reynolds et al., 2008)

located_in nucleus

inferred from direct assay

(Noureddine et al., 2002)

part_of SCF ubiquitin ligase complex

inferred from direct assay

(Wong et al., 2013, Zhang et al., 2013, Reynolds et al., 2008, Donaldson et al., 2004, Bocca et al., 2001)

inferred from mutant phenotype

(Roberts et al., 2012)

inferred from direct assay

(Noureddine et al., 2002)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Roc1a in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Roc1a

View exonic expression by tissue for Dmel\Roc1a

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0025638

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31014/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0025638

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0025638

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0025638

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG16982

Flygut - An atlas of the Drosophila adult midgut

FBgn0025638

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 2 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 15 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Roc1a

Transgenic constructs containing regulatory region of Roc1a

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(1)G1

(Noureddine et al., 2002)

Inferred from location ( 14 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^insc-Mz1407

Roc1a^GD8596

lethal, with Scer\GAL4^pnr-MD237

Roc1a^GD8596

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

Roc1a^GD8596

viable, with Scer\GAL4^tin.CΔ4

Roc1a^GD8596

viable, with Scer\GAL4^{VP16.nanos.UTR}

Other Phenotypes

Allele

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

Roc1a^GD8596

increased cell number | adult stage | progressive | nutrition conditional, with Scer\GAL4^VGlut1-OK371

Roc1a^GD8596

Phenotype manifest in

Allele

adult hemolymph | nutrition conditional, with Scer\GAL4^hs.PB

Roc1a^GD8596

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

Roc1a^GD8596

Johnston organ | P-stage | somatic clone, with Scer\GAL4^Act.PU

Roc1a^GD8596

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

Roc1a^GD8596

mechanosensory neuron of leg campaniform sensillum | increased number | nutrition conditional, with Scer\GAL4^VGlut1-OK371

Roc1a^GD8596

scolopidium | P-stage | somatic clone, with Scer\GAL4^Act.PU

Roc1a^GD8596

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (16)

Hsap\RBX1

14 of 14

Yes

4 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\RLIM

1 of 14

Hsap\RNF24

1 of 14

Yes

Hsap\RNF38

1 of 14

Hsap\RNF44

1 of 14

Hsap\RNF122

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (12)

Rnor\Rbx1

12 of 14

Yes

4 of 14

3 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Rnf139

1 of 14

Mus musculus (laboratory mouse) (13)

Mmus\Rbx1

13 of 14

Yes

3 of 14

3 of 14

2 of 14

2 of 14

Yes

Mmus\Amfr

1 of 14

Mmus\Ltn1

1 of 14

Mmus\Map3k1

1 of 14

Yes

Mmus\Rlim

1 of 14

Mmus\Rnf24

1 of 14

Yes

Mmus\Rnf38

1 of 14

Mmus\Rnf122

1 of 14

Yes

Mmus\Rnf139

1 of 14

Xenopus tropicalis (Western clawed frog) (10)

Xtro\rbx1

7 of 13

Yes

3 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (14)

Drer\rbx1

14 of 14

Yes

4 of 14

2 of 14

1 of 14

1 of 14

Yes

Drer\rlim

1 of 14

Drer\rnf6

1 of 14

Drer\rnf24

1 of 14

Yes

Drer\rnf38

1 of 14

Drer\rnf44

1 of 14

Drer\rnf122

1 of 14

Yes

Drer\rnf139

1 of 14

Drer\rnf165b

1 of 14

Drer\ttc3

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (12)

Cele\rbx-1

14 of 14

Yes

4 of 14

3 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\plr-1

1 of 14

Cele\sel-11

1 of 14

Cele\T22B2.1

1 of 14

Yes

Cele\toe-4

1 of 14

Cele\Y54E10A.11

1 of 14

Cele\ZK637.14

1 of 14

Anopheles gambiae (African malaria mosquito) (12)

Agam\AgaP_AGAP005202

11 of 12

Yes

4 of 12

3 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Agam\AgaP_AGAP013357

1 of 12

Arabidopsis thaliana (thale-cress) (95)

Atha\RBX1

13 of 13

Yes

Atha\AT3G42830

10 of 13

Yes

3 of 13

3 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AT1G60360

1 of 13

Atha\AT1G68070

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AT3G48030

1 of 13

Atha\AT3G54780

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AT5G60710

1 of 13

Yes

Atha\AT5G67120

1 of 13

Atha\ATL1F

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\RDUF1

1 of 13

Atha\RDUF2

1 of 13

Atha\RHA1A

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\RIN2

1 of 13

Atha\RIN3

1 of 13

Atha\RING1

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (5)

Scer\HRT1

11 of 13

Yes

2 of 13

1 of 13

1 of 13

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (5)

Spom\rbx1

12 of 12

Yes

Spom\apc11

2 of 12

Spom\SPBC21D10.09c

1 of 12

Spom\SPBP4H10.07

1 of 12

Yes

Spom\SPCC4G3.12c

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Roc1a. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (6)

10 of 13

6 of 13

4 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RBX1; ring-box 1

14 of 14

603814

RNF7; ring finger protein 7

4 of 14

603863

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Roc1a - Cul1

anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, western blot, Identification by mass spectrometry

(Zhang et al., 2013, Reynolds et al., 2008, Donaldson et al., 2004)

Roc1a - Cul2

anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation, Identification by mass spectrometry

(Reynolds et al., 2008, Donaldson et al., 2004)

Roc1a - Cul3

anti tag coimmunoprecipitation, western blot, anti tag western blot

(Zhou et al., 2015, Reynolds et al., 2008)

Roc1a - Cul4

anti tag coimmunoprecipitation, western blot

(Reynolds et al., 2008)

Roc1a - TER94

anti tag coimmunoprecipitation, anti tag western blot

(Zhang et al., 2013)

Roc1a - Ufd1

anti tag coimmunoprecipitation, anti tag western blot, pull down, western blot

(Zhang et al., 2013)

Roc1a - slmb

anti tag coimmunoprecipitation, western blot, anti tag western blot

(Wong et al., 2013, Zhang et al., 2013)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Lee et al., 2024)

suppressible

(Donaldson et al., 2004)

Uch

suppressible

Roc1a

(Lee et al., 2024)

External Data

Subunit Structure (UniProtKB)

(UniProt, Q9W5E1 )

Linkouts

BioGRID - A database of protein and genetic interactions.

57582

DroID - A comprehensive database of gene and protein interactions.

FBgn0025638

MIST (protein-protein) - An integrated Molecular Interaction Database

31014

Pathways

Signaling Pathways (FlyBase)

NEGATIVE REGULATORS OF WNT-TCF SIGNALING PATHWAY
NEGATIVE REGULATORS OF INSULIN-LIKE RECEPTOR SIGNALING PATHWAY
NEGATIVE REGULATORS OF HEDGEHOG SIGNALING PATHWAY

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9W5E1

Class of Gene

gene_with_edited_transcript

(Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-0

Cytogenetic map

1B13-1B13

Sequence location

X:647,304..648,597 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

1B13-1B13

Limits computationally determined from genome sequence between P{EP}Dredd^EP1412&P{EP}Suv4-20^EP1216a and P{EP}sdk^EP369

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-0

(FlyBase, 2016)

1-0.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (10)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

35495

w^*; P{Roc1a.V5}3

34047

w^*; P{UAS-Roc1a.D}8.1

26607

w^* P{EP}Roc1a^G824/FM6, w^*

96300

y¹ w^*; P{TKO-UAS.GS05952}attP40/CyO

96366

y¹ v¹; P{SAM.dCas9.GS06019}attP40/CyO

602322

y¹ TI{KozakGAL4}Roc1a^{CR71064-KO-kG4} w^*/FM7h

32362

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00353}attP2

NIG-Fly

HMS00353

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00353}attP2

VDRC

v106315

P{KK108885}VIE-260B

v32399

w¹¹¹⁸; P{GD8596}v32399

Genomic Clones (11)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (42)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

SD23839

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0025638

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0025638

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

S2 cells are treated with dsRNA made from templates generated with primers directed against this gene to assess effect on degradation of dup and geminin genes.

(Higa et al., 2003)

Roc1a is an essential gene.

(Noureddine et al., 2001)

Identification: cDNA screen for secreted and transmembrane proteins expressed during embryogenesis.

(Kopczynski et al., 1998)

Relationship to Other Genes

Source for database merge of

Source for merge of: EG:115C2.11 BEST:CK01110

(Bayraktaroglu, 2000.11.2)

Source for merge of: Roc1a anon- EST:Posey65

(Ashburner, 2004.6.30)

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: EG:115C2.11 CG16982

(Gong, 1999.11)

Source for identity of: Roc1a EG:115C2.11

(Duronio, 2001.3.7)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (20)

Reported As

Symbol Synonym

BEST:CK01110

CG16982

(Hosono et al., 2015, Schertel et al., 2013, Ying et al., 2011, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Ashburner, 2004.6.30, Duronio, 2001.3.7, Duronio, 2001.3.7, Benos, 2000.12.13)

CK01110

(Kopczynski et al., 1998)

EG:115C2.11

(Bolshakov et al., 2002, Benos et al., 2001)

RBX1

(Lee et al., 2024)

ROC1

(Ozturk et al., 2013, Duronio, 2001.3.7)

Rbx1

(Peng et al., 2022, Kohzaki, 2018, Kohzaki et al., 2018, Nayak et al., 2018, Das et al., 2002)

Rbx1/Roc

(Jin and Harper, 2002)

Rbx1a

(Duarte et al., 2024)

Roc

(Kroeger et al., 2013)

Roc1

(Gujar and Wang, 2025, Kohzaki, 2018)

Roc1a

(Lawson et al., 2025, Lawson et al., 2025, Lawson et al., 2025, Lawson et al., 2025, Duarte et al., 2024, Brener et al., 2023, Kumar and Baker, 2022, Barbosa et al., 2021, Pang et al., 2021, Gene Disruption Project members, 2018-, Podolsky et al., 2018, Ketosugbo et al., 2017, Pan et al., 2017, Transgenic RNAi Project members, 2017-, Zhou et al., 2015, Gonzalez et al., 2014, Skwarek et al., 2014, Bonke et al., 2013, Kroeger et al., 2013, Schertel et al., 2013, Strutt et al., 2013, Wong et al., 2013, Zhang et al., 2013, Zhang et al., 2013, Gurudatta et al., 2012, Roberts et al., 2012, Tokusumi et al., 2012, Du et al., 2011, Du et al., 2011, Lindquist et al., 2011, Rohn et al., 2011, Toku et al., 2011, Ying et al., 2011, Kaplan et al., 2010, Rogers et al., 2009, Alexandre et al., 2008, Beller et al., 2008, Reynolds et al., 2008, Straub et al., 2008, Langille and Clark, 2007, Jiang, 2006)

Roc1α

(Ogden et al., 2004)

anon-EST:Posey65

dRbx1

(Bocca, 2001.8.27, Bocca et al., 2001, Muzzopappa et al., 2001)

dRoc1

(Heriche et al., 2003)

dRoc1a

(Noureddine et al., 2001)

roc1a

(Li et al., 2016)

Name Synonyms

Regulator of cullins 1a

Roc1a

SCF-βTrCP complex

(Marthiens and Basto, 2020)

Secondary FlyBase IDs

FBgn0025527
FBgn0025705

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 78 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

31014

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9W5E1

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9W5E1

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9W5E1

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0025638

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31014/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0025638

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0025638

FlyMine - An integrated database for Drosophila genomics

FBgn0025638

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG16982

MARRVEL_MODEL - MARRVEL (model organism gene)

31014

Linkouts

BioGRID - A database of protein and genetic interactions.

57582

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0025638

DroID - A comprehensive database of gene and protein interactions.

FBgn0025638

DRSC - Results frm RNAi screens

FBgn0025638

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Roc1a_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0025638

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0025638

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG16982

Flygut - An atlas of the Drosophila adult midgut

FBgn0025638

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC007016

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (protein-protein) - An integrated Molecular Interaction Database

31014

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9W5E1

References (111)

The following external sites may use different assemblies or annotations than FlyBase.

-0.99

Report Sections