FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Roc1a, Cul1
FlyBase ID
FBig0000097946
Interaction Network
Interactions Browser links
Roc1a network
Cul1 network
Reported Interactions
FBrf0180504-1.coIP.WB
Reference
Donaldson et al., 2004
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Roc1a 

Roc1a

bait

Flag-tag

Cul1 

CUL-1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Roc1a efficiently immunoprecipitates both neddylated and unmodified CUL-1

(Donaldson et al., 2004)

Source was 4-8 hr embryos; bait produced from transgenic construct; prey produced from endogenous gene.

(Donaldson et al., 2004)
FBrf0180504-4.coIP.MS
Reference
Donaldson et al., 2004
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Roc1a 

Roc1a

bait

Flag-tag

Cul1 

CUL-1

prey

13 unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryo lysates; bait produced from transgenic construct; prey produced from endogenous gene.

(Donaldson et al., 2004)
FBrf0205823-1.coIP.WB
Reference
Reynolds et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Roc1a 

ROC1A

bait

FLAG or V5 tag

Cul1 

Cul1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Reynolds et al., 2008)
FBrf0205823-7.coIP.WB
Reference
Reynolds et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Roc1a 

ROC1A

bait

V5 tag

Cul1 

Cul1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Reynolds et al., 2008)
FBrf0221943-15.coIP.WB
Reference
Zhang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Cul1 

Cul1

bait

FLAG tag

Roc1a 

Roc1a

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhang et al., 2013)

Roc1a interacts more strongly with lin19 (Cul1) than slmb in this assay.

(Zhang et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)