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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000097690
Interaction Network
Interactions Browser links
Lar network
dlp network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0191320-2.AT.TV
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Lar 

LAR

bait

FLAG tag

Lar 
dlp 

Dlp

prey

Fc, Alkaline phosphatase tags

dlp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Lar 
ectodomain and transmembrane domain
sufficient binding region
dlp 
ectodomain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in vertebrate cell line; prey produced from transfected construct.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

dlp ectodomain binds S2 cells expressing Lar, but not to untransfected S2 cells.

Purified Sdc fusion protein was tested for its ability to bind live S2 cells expressing Lar protein.

FBrf0191320-4.PD.ELISA
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Lar 

LAR

bait

Fc tag

Lar 
dlp 

Dlp

prey

alkaline phosphatase tag

dlp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Lar 
Ig-like domains
sufficient binding region
dlp 
ectodomain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in vertebrate cell line; prey produced as a recombinant fusion protein in vertebrate cell line.

Lar binds to dlp (Kd = 7.8nM) more strongly than to Sdc (Kd = 12.8nM).

Removal of dlp glycosylation by enzymatic treatment disrupts the interaction.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)