An S120 extract, in which plasma membrane and internal membrane compartments were removed by centrifugation, was subjected to Optiprep density gradient fractionation.
Source was larvae of transgenic fly line; proteins produced from endogenous gene or from tagged transgenic construct.
Source was larvae of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Weak binding. Test for interaction using a full length dlp construct was not reported.