Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

weak interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Coimmunoprecipitation from "high-speed lysates" depleted of mature centrosomes.

Source was embryos of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

Sas-4-containing complexes were affinity-purified, then subjected to immunoprecipitation by antibodies to cnn protein.

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Coimmunoprecipitation from "high-speed lysates" depleted of mature centrosomes.

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

Characterization of Sas-4 complexes shows that Sas-4 and cnn cosediment in a narrow range of fractions at 7S.

Coimmunoprecipitation from "high-speed lysates" depleted of mature centrosomes.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

Free tubulin can disrupt the interaction of Sas-4 with cnn; mutation of the Sas-4 tubulin binding domain prevents this competition.

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

cnn coimmunoprecipitates and co-fractionates with Sas-4 as part of the S-CAP complex (centrosomal scaffold).

Sas-4 complexes were immunoprecipitated from "high-speed lysates" depleted of mature centrosomes and different Sas-4-containing complexes were fractionated on a sucrose gradient.

This interaction is not affected by mutations to the β9-10 surface of the TCP domain: E792A, V800A/I802A or L841K.

Source was cell extract of C131 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Constructs were expressed in acentrosomal cell line derived from Sas-4 null homozygous mutant flies.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

The N-terminal regions of cnn and Sas-4 bind each other. The N-terminal region of one also binds the central region of the other. However, the central regions of cnn and Sas-4 do not interact with each other.