Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast LexA-BD/B42-AD

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

The interaction of tub with dl is weaker than the interaction of cact with dl.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from ovary cDNA expression library).

5 out of 20 dl-interacting hits in this screen represented tub cDNAs.

Two-hybrid system: yeast LexA-BD/B42-AD

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

Purified tub was assayed for its ability to block the binding of purified dl protein to a [32]P labeled DNA fragment of the zen promoter, as determined by gel shift assays. tub alone did not bind to zen DNA.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast LexA-BD/B42-AD

Two-hybrid system: yeast LexA-BD/B42-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from ovarian cDNA expression library).