FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Hira, Trl
FlyBase ID
FBig0000097796
Interaction Network
Interactions Browser links
Hira network
Trl network
Reported Interactions
FBrf0195269-1.coIP.WB
Reference
Nakayama et al., 2007
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 12embryonic stage 17embryonic stage 13embryonic stage 14embryonic stage 15dorsal closure stageembryonic stage 16
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Trl 

GAGA

bait
Hira 

HIRA

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of transgenic fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Nakayama et al., 2007)
FBrf0195269-2.coIP.WB
Reference
Nakayama et al., 2007
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 12embryonic stage 17embryonic stage 13embryonic stage 14embryonic stage 15dorsal closure stageembryonic stage 16
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hira 

HIRA

bait
Trl 

GAGA

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of transgenic fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Nakayama et al., 2007)
FBrf0195269-3.coIP.WB
Reference
Nakayama et al., 2007
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 12embryonic stage 17embryonic stage 13embryonic stage 14embryonic stage 15dorsal closure stageembryonic stage 16
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Trl 

GAGA

bait

FLAG tag

Hira 

HIRA

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Nakayama et al., 2007)
FBrf0195269-4.coIP.WB
Reference
Nakayama et al., 2007
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 12embryonic stage 17embryonic stage 13embryonic stage 14embryonic stage 15dorsal closure stageembryonic stage 16
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hira 

HIRA

bait

FLAG tag

Trl 

GAGA

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Nakayama et al., 2007)
FBrf0219981-23.coIP.WB
Reference
Nakayama et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 17blastoderm stagegastrula stageembryonic stage 8dorsal closure stageembryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic cycle 9embryonic stage 2late extended germ band stageembryonic stage 5embryonic stage 11embryonic stage 3embryonic stage 12embryonic stage 15embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7embryonic stage 16
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Trl 

GAGA

bait
Hira 

HIRA

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

(Nakayama et al., 2012)
FBrf0260924-8.coIP.WB
Reference
Zhang et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Trl 

Gaf

bait

FLAG tag

Trl 
Hira 

Hira

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Trl 
BTB domain
necessary binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhang et al., 2024)
FBrf0260924-9.coIP.WB
Reference
Zhang et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Hira 

Hira

bait

FLAG tag

Hira 
Trl 

Gaf

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Hira 
N-terminal domain
necessary binding region
aa 1-400

coordinates relative to Hira-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhang et al., 2024)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)