FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
DCP1, pcm
FlyBase ID
FBig0000097825
Interaction Network
Interactions Browser links
DCP1 network
pcm network
Reported Interactions
FBrf0220119-1.coIP.WB
Reference
Braun et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
pcm 

XRN1

bait

V5 tag

pcm 
pcm 
pcm 
DCP1 

DCP1

prey

HA tag

DCP1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pcm 
DCP1-binding motif
sufficient binding region
aa 1323-1355

\'DBM\'

DCP1 
EVH1 domain
mutation disrupting interaction
aa 96aa 34

Y34A

R96A

pcm 
DCP1-binding motif
mutation disrupting interaction
aa 1335-1338aa 1341aa 1342aa 1345aa 1349

M1335A, L1338A double mutant

P1341A

P1342A

W1345A

A1349P

pcm 
DCP1-binding motif
mutation decreasing interaction
aa 1339

P1339A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Braun et al., 2012)

The pcm-Dcp1 interaction persists in the presence of RNaseA, and is thus not mediated by RNA.

(Braun et al., 2012)
FBrf0220119-3.coIP.WB
Reference
Braun et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
pcm 

XRN1

bait
DCP1 

DCP1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Braun et al., 2012)
FBrf0220119-4.coIP.WB
Reference
Braun et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
DCP1 

DCP1

bait

HA tag

DCP1 
pcm 

XRN1

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
DCP1 
EVH1 domain
sufficient binding region
aa 1-127
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Braun et al., 2012)
FBrf0220119-5.PD.MW
Reference
Braun et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
DCP1 

DCP1

bait

GST tag

DCP1 
pcm 

XRN1

prey

His tag

pcm 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
DCP1 
EVH1 domain
sufficient binding region
aa 1-127
pcm 
DCP1-binding motif
sufficient binding region
aa 1323-1355

\'DBM\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Braun et al., 2012)
FBrf0220119-6.NMR
Reference
Braun et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
DCP1 

DCP1

bait
DCP1 
pcm 

XRN1

prey
pcm 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
DCP1 
EVH1 domain
sufficient binding region
aa 1-127
pcm 
DCP1-binding motif
sufficient binding region
aa 1323-1355

\'DBM\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Braun et al., 2012)
FBrf0220119-7.coIP.WB
Reference
Braun et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
DCP1 

DCP1

bait

GFP tag

DCP1 
pcm 

XRN1

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
DCP1 
EVH1 domain
mutation disrupting interaction
aa 34

Y34A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Braun et al., 2012)

Disruption of the Dcp1-pcm interaction by the Dcp1 Y34A mutation does not disrupt Dcp1-Dcp2 or Dcp1-Ge-1 interactions.

(Braun et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)