Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2R+ cell extract.
Myristoylation of Arf79F is achieved by coexpression of N-myristoyltransferase in bacterial cells.
Myristoylated and GTPĪ³S-loaded Arf79F was bound to silica beads coated with phospholipid bilayers, then mixed with cell extract.
