Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

The cry-BRWD3 interaction is only detected when yeast cells are grown in light.

BRWD3 promotes light-dependent degradation of cry. 25 other F-box proteins tested do not seem to mediate light-dependent cry degradation.

Two-hybrid system: yeast LexA-BD/B42-AD

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected Sf21 cell system; prey produced as a recombinant fusion protein in baculovirus-infected Sf21 cell system.

The cry-BRWD3 interaction is light-dependent.

cry binds to jet more strongly than to BRWD3.

The reaction mixtures were incubated in the dark or under light for 12.5 minutes.

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected Sf21 cell system; prey produced as a recombinant fusion protein in baculovirus-infected Sf21 cell system.

The cry-BRWD3 interaction is light-dependent.

The reaction mixtures were incubated in the dark or under light for 12.5 minutes.

The cry-BRWD3 interaction is light-dependent.

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected Sf21 cell system; prey produced as a recombinant fusion protein in baculovirus-infected Sf21 cell system.

The reaction mixtures were incubated in the dark or under light for 12.5 minutes.