FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
aos, spi
FlyBase ID
FBig0000097935
Interaction Network
Interactions Browser links
aos network
spi network
Reported Interactions
FBrf0179757-1.SPR
Reference
Klein et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
aos 

Argos

bait

His tag

spi 

Spitz

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait and prey produced and purified from transfected constructs.

(Klein et al., 2004)
FBrf0179757-2.SPR
Reference
Klein et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
spi 

Spitz

bait

His tag

spi 
aos 

Argos

prey

His tag

aos 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
aos 
C-terminal domain
sufficient binding region
aa 224-444
spi 
EGF motif
sufficient binding region
aa 67-128
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait and prey produced and purified from transfected constructs.

(Klein et al., 2004)
FBrf0179757-5.CS
Reference
Klein et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
aos 

Argos

neutral component
aos 

Argos

neutral component
aos 

Argos

neutral component
spi 

Spitz

neutral component
spi 

Spitz

neutral component
spi 

Spitz

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced and purified from transfected constructs.

(Klein et al., 2004)

aos and spi form a 1:1 complex in solution

(Klein et al., 2004)
FBrf0190214-2.SPR
Reference
Miura et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
spi 

sSpi

bait
spi 
aos 

Aos

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
spi 
soluble domain
sufficient binding region
aa 1-128
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in S2 cell line; prey produced as a recombinant fusion protein.

(Miura et al., 2006)

Interaction of spi with aos is not affected by mutation of the spi palmitoylation site at residue Cys29.

(Miura et al., 2006)

Positive control.

(Miura et al., 2006)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)