FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Sema1a, pbl
FlyBase ID
FBig0000097937
Interaction Network
Interactions Browser links
Sema1a network
pbl network
Reported Interactions
FBrf0219982-1.coIP.WB
Reference
Jeong et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
pbl 

Pbl

bait

HA tag

pbl 
Sema1a 

Sema-1a

prey

Myc tag

Sema1a 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sema1a 
intracellular domain
sufficient binding region
SFPSSCRIQQEPKLLPQVEEVTYAEPVLLPQPPPPNKMHSPKNTLRKPPMHQMHQGPNSE
pbl 
BRCT domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jeong et al., 2012)

pbl interacts strongly with Sema-1a, but only weakly with plexA, and not at all with plexB or otk.

(Jeong et al., 2012)
FBrf0219982-6.coIP.WB
Reference
Jeong et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Sema1a 

Sema-1a

bait
pbl 

Pbl

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jeong et al., 2012)

pbl and RhoGAPp190 compete for binding to Sema-1a.

(Jeong et al., 2012)
FBrf0236763-6.coIP.WB
Reference
Jeong et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
pbl 

pbl

bait

HA tag

Sema1a 

Sema-1a

prey

Myc tag

Sema1a 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sema1a 
intracellular domain
sufficient binding region
aa 685-826

coordinates relative to Sema1a-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jeong et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)