FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Crag, Rab11
FlyBase ID
FBig0000097969
Interaction Network
Interactions Browser links
Crag network
Rab11 network
Reported Interactions
FBrf0220111-1.coIP.WB
Reference
Xiong et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Rab11 

Rab11

bait

HA tag

Crag 

Crag

prey

FLAG tag

Crag 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Crag 
DENN domains
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Xiong et al., 2012)

Crag preferentially binds a dominant-negative version of Rab11 that mimics the GDP-bound state.

(Xiong et al., 2012)
FBrf0220111-2.ENZ
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Crag 

Crag

enzyme

GST tag

Crag 

Crag

enzyme

GST tag

Rab11 

Rab11

enzyme target

fluorescent GDP label

Rab11 

Rab11

enzyme target

fluorescent GDP label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)