FB2021_02, released April 13, 2021

Physical Interaction report

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General Information
Interaction Type
physical association
Interacting Genes
Ada2b, p53
FlyBase ID
FBig0000097991
Interaction Network
Interactions Browser links
Ada2b network
p53 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0159212-32.PD.WB
Reference
Kusch et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
p53 

Dmp53

bait

GST tag

Ada2b 

dAda2b

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Ada2b, but not Ada2a, interacts with p53.

(Kusch et al., 2003)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell nuclear extract.

(Kusch et al., 2003)
FBrf0221014-3.coIP.WB
Reference
Pankotai et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Ada2b 

dADA2b

bait

FLAG tag

p53 

p53

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Long and short isoforms of Ada2b can associate with p53.

(Pankotai et al., 2013)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Pankotai et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)