FlyBase Gene Report: Dmel\Ada2b

(Fisher et al., 2012)

embryonic stage 7 -- 8

(Fisher et al., 2012)

embryonic stage 9 -- 10

(Fisher et al., 2012)

embryonic stage 11 -- 12

larval ventral nerve cord

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

pupal stage

organism

(Zsindely et al., 2009)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Muratoglu et al., 2003)

inferred from direct assay

(Weake et al., 2011, Guelman et al., 2006)

located_in polytene chromosome

inferred from direct assay

(Nagy et al., 2010, Kusch et al., 2003, Muratoglu et al., 2003)

part_of SAGA complex

inferred from direct assay

(Torres-Zelada et al., 2019, Weake et al., 2011, Guelman et al., 2006)

inferred from physical interaction with FLYBASE:Spt3; FB:FBgn0037981

(Kusch et al., 2003)

inferred from physical interaction with FLYBASE:Nipped-A; FB:FBgn0053554

(Kusch et al., 2003)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Ada2b in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Ada2b

View exonic expression by tissue for Dmel\Ada2b

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0037555

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40966/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0037555

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0037555

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0037555

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9638

Flygut - An atlas of the Drosophila adult midgut

FBgn0037555

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 10 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 22 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Ada2b

Transgenic constructs containing regulatory region of Ada2b

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(3R)Ada2b^d52

(Pankotai et al., 2005)

Df(3R)dsx5

(Qi et al., 2004)

Inferred from location ( 8 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal (with Ada2b¹)

Ada2b^d842

lethal (with Ada2b^d842)

Ada2b¹

lethal | pharate adult stage

lethal | pharate adult stage (with Df(3R)Ada2b^d52)

lethal | pupal stage P5

lethal | pupal stage P5 (with Df(3R)Ada2b^d52)

lethal - all die before end of pupal stage

Ada2b^d842

lethal - all die before end of pupal stage | recessive

partially lethal - majority die

Ada2b^HD-5

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Ada2b^GD14276

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Ada2b^GD14276

viable, with Scer\GAL4^Mef2.PR

Ada2b^GD14276

viable, with Scer\GAL4^tin.CΔ4

Ada2b^GD14276

Sterility

Allele

female sterile | germline clone | recessive

fertile, with Ada2b¹/Ada2b^d842

Ada2b^PA

fertile, with Ada2b¹/Ada2b^d842, Ada2b^PA

Ada2b^PB

fertile, with Ada2b¹/Ada2b^d842, Ada2b^PB

Ada2b^PA

fertile (with Ada2b¹), with Ada2b^PA

Ada2b^d842

fertile (with Ada2b¹), with Ada2b^PA, Ada2b^PB

Ada2b^d842

fertile (with Ada2b^d842), with Ada2b^PA

Ada2b¹

fertile (with Ada2b^d842), with Ada2b^PA, Ada2b^PB

Ada2b¹

male fertile, with Scer\GAL4^bam.PU

Ada2b^GL00243

Other Phenotypes

Allele

abnormal cell migration | oogenesis | somatic clone

Ada2b¹

abnormal locomotor rhythm | adult stage, with Scer\GAL4^Pdf.PU

Ada2b^NIG.9638R

abnormal locomotor rhythm | adult stage, with Scer\GAL4^tim.PE

Ada2b^NIG.9638R

abnormal neuroanatomy

Ada2b¹

abnormal neurophysiology | larval stage

Ada2b¹

abnormal neurophysiology | larval stage, with Scer\GAL4^egr-GAL4

Ada2b^JF01305

abnormal neurophysiology | larval stage, with Scer\GAL4^NP6293

Ada2b^JF01305

some die during pupal stage

Ada2b^d842

some die during pupal stage, with Scer\GAL4^pnr-MD237

Ada2b^GD14276

some die during pupal stage | recessive

visible, with Scer\GAL4^pnr-MD237

Ada2b^GD14276

Phenotype manifest in

Allele

axon & photoreceptor cell R2

Ada2b¹

axon & photoreceptor cell R3

Ada2b¹

axon & photoreceptor cell R4

Ada2b¹

axon & photoreceptor cell R5

Ada2b¹

border follicle cell | somatic clone

Ada2b¹

embryonic/larval neuromuscular junction | larval stage

Ada2b¹

embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4^egr-GAL4

Ada2b^JF01305

embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4^NP6293

Ada2b^JF01305

glial cell

Ada2b¹

larval abdominal segment, with Scer\GAL4^egr-GAL4

Ada2b^JF01305

NMJ bouton | increased number | larval stage, with Scer\GAL4^egr-GAL4

Ada2b^JF01305

presynaptic active zone | increased number | larval stage, with Scer\GAL4^egr-GAL4

Ada2b^JF01305

trichogen cell, with Scer\GAL4^pnr-MD237

Ada2b^GD14276

wing disc | conditional

Ada2b^unspecified

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (3)

Hsap\TADA2B

13 of 14

Yes

Hsap\TADA2A

5 of 14

Hsap\DTNA

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (2)

Rnor\Tada2b

13 of 14

Yes

Rnor\Tada2a

5 of 14

Mus musculus (laboratory mouse) (2)

Mmus\Tada2b

12 of 14

Yes

Mmus\Tada2a

5 of 14

Xenopus tropicalis (Western clawed frog) (3)

Xtro\tada2b

7 of 13

Yes

Xtro\tada2a

2 of 13

Xtro\espn

1 of 13

Danio rerio (Zebrafish) (2)

Drer\tada2b

13 of 14

Yes

Drer\tada2a

5 of 14

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\ada-2

8 of 14

Yes

Cele\sqst-5

1 of 14

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP003109

10 of 12

Yes

Arabidopsis thaliana (thale-cress) (3)

Atha\ADA2A

11 of 13

Yes

Atha\ADA2B

10 of 13

Atha\AT4G09450

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\ADA2

10 of 13

Yes

Scer\FUN19

1 of 13

Scer\YOR338W

1 of 13

Schizosaccharomyces pombe (Fission yeast) (3)

Spom\ada2

10 of 12

Yes

Spom\laf1

1 of 12

Yes

Spom\laf2

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Ada2b. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

Ada2a

7 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Ada2b^NIG.9638R

ameliorates amyotrophic lateral sclerosis

modeled by Vap33^P58S.cRa.UAS

(Deivasigamani et al., 2014)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TADA2B; transcriptional adaptor 2B

13 of 14

608790

TADA2A; transcriptional adaptor 2A

5 of 14

602276

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Ada2b

two hybrid, anti tag coimmunoprecipitation, anti tag western blot

(Torres-Zelada et al., 2019, Pankotai et al., 2013)

Ada2b - Ada1-2

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011, Weake et al., 2009)

Ada2b - Ada2a

two hybrid, anti tag coimmunoprecipitation, peptide massfingerprinting, anti bait coimmunoprecipitation, western blot, multidimensional protein identification technology, affinity chromatography technology, molecular sieving, anti tag western blot

(Lebedeva et al., 2004, Muratoglu et al., 2003)

Ada2b - Ada3

(Soffers et al., 2019, Torres-Zelada et al., 2019, Pankotai et al., 2013, Weake et al., 2011, Weake et al., 2009, Ciurciu et al., 2006, Kusch et al., 2003, Muratoglu et al., 2003)

Ada2b - Alh

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - Atac3

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - Atxn7

anti tag coimmunoprecipitation, western blot, peptide massfingerprinting, molecular sieving

(Mohan et al., 2014, Weake et al., 2011, Weake et al., 2009)

Ada2b - Brf

anti tag coimmunoprecipitation, peptide massfingerprinting

(Popova et al., 2018)

Ada2b - Bug22

(Weake et al., 2011)

Ada2b - CG11970

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - CG3815

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - CG42724

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - CG45071

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - CG6470

anti tag coimmunoprecipitation, peptide massfingerprinting

(Shokri et al., 2019)

Ada2b - CTCF

(Weake et al., 2011)

Ada2b - CrebB

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - Deaf1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - Dp

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - E2f2

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - ERR

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - Ets65A

anti tag coimmunoprecipitation, peptide massfingerprinting

(Shokri et al., 2019)

Ada2b - Ets97D

(Weake et al., 2011)

Ada2b - FoxK

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - GATAd

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - GATAe

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - HIP-R

experimental knowledge based

(Guruharsha et al., 2011)

Ada2b - Hcf

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - Hr3

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - Hr78

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - Hsf

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - Jra

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - M1BP

experimental knowledge based

(Guruharsha et al., 2011)

Ada2b - Max

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - Mef2

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - Myb

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - Nipped-A

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, peptide massfingerprinting

(Popova et al., 2018, Weake et al., 2011, Weake et al., 2009, Kusch et al., 2003)

Ada2b - Noc1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - Pbp45

molecular sieving, western blot

(Popova et al., 2018)

Ada2b - Polr2A

(Lebedeva et al., 2004)

Ada2b - Ptx1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - Saf6

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011, Weake et al., 2009)

Ada2b - Sf3b3

anti tag coimmunoprecipitation, peptide massfingerprinting

(Stegeman et al., 2016)

Ada2b - Sf3b5

anti tag coimmunoprecipitation, peptide massfingerprinting, two hybrid

(Stegeman et al., 2016)

Ada2b - Sgf11

anti tag coimmunoprecipitation, peptide massfingerprinting, anti tag western blot

(Weake et al., 2011, Weake et al., 2009, Weake et al., 2008)

Ada2b - Sgf29

anti tag coimmunoprecipitation, peptide massfingerprinting, western blot, affinity chromatography technology, molecular sieving, multidimensional protein identification technology

(Soffers et al., 2019, Weake et al., 2011, Weake et al., 2009, Weake et al., 2008)

Ada2b - Ski8

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - Spt20

anti tag coimmunoprecipitation, peptide massfingerprinting

(Stegeman et al., 2016, Weake et al., 2011, Weake et al., 2009)

Ada2b - Spt3

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, peptide massfingerprinting, two hybrid, multidimensional protein identification technology

(Torres-Zelada et al., 2019, Stegeman et al., 2016, Weake et al., 2011, Weake et al., 2009, Weake et al., 2008, Guelman et al., 2006, Kusch et al., 2003)

Ada2b - Spt7

anti tag coimmunoprecipitation, peptide massfingerprinting, two hybrid, multidimensional protein identification technology

(Torres-Zelada et al., 2019, Weake et al., 2011, Weake et al., 2009)

Ada2b - Stat92E

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - Taf10

anti tag coimmunoprecipitation, peptide massfingerprinting

(Lebedeva et al., 2004, Kusch et al., 2003, Muratoglu et al., 2003)

Ada2b - Taf10b

(Weake et al., 2011, Weake et al., 2009)

Ada2b - Taf12

anti tag coimmunoprecipitation, peptide massfingerprinting, two hybrid

(Torres-Zelada et al., 2019, Weake et al., 2011, Weake et al., 2009)

Ada2b - Taf4

molecular sieving, western blot, anti bait coimmunoprecipitation, ion exchange chromatography

(Lebedeva et al., 2004, Muratoglu et al., 2003)

Ada2b - Taf5

anti tag coimmunoprecipitation, peptide massfingerprinting, ion exchange chromatography, molecular sieving, western blot, anti bait coimmunoprecipitation

(Kusch et al., 2003)

Ada2b - Taf9

(Weake et al., 2011, Weake et al., 2009, Lebedeva et al., 2004, Kusch et al., 2003, Muratoglu et al., 2003)

Ada2b - Tbp

anti tag coimmunoprecipitation, peptide massfingerprinting

(Lebedeva et al., 2004, Muratoglu et al., 2003)

Ada2b - bab2

(Weake et al., 2011)

Ada2b - br

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - cg

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - chinmo

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - chn

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - ci

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - ct

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - d4

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - dve

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - e(y)2

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011, Kurshakova et al., 2009, Weake et al., 2009, Kurshakova et al., 2007)

Ada2b - fkh

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - fru

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - ftz-f1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - gro

anti tag coimmunoprecipitation, peptide massfingerprinting

(Shokri et al., 2019)

Ada2b - kay

(Weake et al., 2011)

Ada2b - ken

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - l(3)mbt

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - not

anti tag coimmunoprecipitation, western blot, peptide massfingerprinting

(Cloud et al., 2019, Weake et al., 2011, Weake et al., 2009)

Ada2b - oc

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - p53

anti tag coimmunoprecipitation, anti tag western blot, pull down, western blot

(Pankotai et al., 2013, Kusch et al., 2003)

Ada2b - peb

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - pho

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - pnr

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - pps

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - rib

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - stc

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

Ada2b - sug