Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct in muscle (Mef2-GAL4); prey produced from endogenous gene.
This interaction was observed in muscle and nervous system but not S2 cells.
Proteins that non-specifically bind FLAG beads were identified in control purifications from untagged extract (OregonR) and excluded from further analysis.
This interaction was observed in muscle and nervous system but not S2 cells.
Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct in nervous system (elav-GAL4); prey produced from endogenous gene.
Proteins that non-specifically bind FLAG beads were identified in control purifications from untagged extract (OregonR) and excluded from further analysis.