Gcn5 interacts with both Ada2a and Ada2b.

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Ada2b, but not Ada2a, copurifies with Taf4, Gcn5 and e(y)1 in a large complex, approximately 2 MDa in size.

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Embryonic nuclear extract was first fractionated on a heparin column, then size fractioned by Superose 6 gel filtration.

Gcn5 interacts with both Ada2a and Ada2b.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast LexA-BD/GAL4-AD

Ada2b and Gcn5 copurify in a large complex of about 1-2 MDa, consistent with the size of the SAGA complex.

Source was nuclear extract of S2 cell line; proteins produced from endogenous gene.

Nuclear extract was first fractionated by anion exchange, then by size exclusion chromatography.

Ada2b, but not Ada2a, interacts with p53.

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Tandem affinity purification of bait protein (Protein A, then CBP purification) and MudPIT identification of prey proteins.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast lexA-BD/GAL4-AD

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Nuclear extract was treated with DNase and RNase.

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

The Drosophila SAGA complex is composed of the following subunits: Gcn5, Ada2b, dik, Sgf29, CG6506, Ada1-2, Spt3, Spt20, Nipped-A, e(y)1 (Taf9), Taf10b, Taf12, wda, Saf6, not, Sgf11, e(y)2 and Atxn7.

Tandem affinity purification of bait and identification of prey by MudPIT.

Source was embryonic nuclei of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

The Gcn5-Ada2b interaction is not affected in Saf6 mutant embryos.

Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct in nervous system (elav-GAL4); prey produced from endogenous gene.

All SAGA subunits were present in purifications from both muscle and neuronal cells.

Proteins that non-specifically bind FLAG beads were identified in control purifications from untagged extract (OregonR) and excluded from further analysis.

Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct in muscle (Mef2-GAL4); prey produced from endogenous gene.

All SAGA subunits were present in purifications from both muscle and neuronal cells.

Proteins that non-specifically bind FLAG beads were identified in control purifications from untagged extract (OregonR) and excluded from further analysis.

Gcn5, Nipped-A, Ada2b, Taf10 and e(y)2 are components of the SAGA/TFTC complex.

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD. The Ada2b-PA isoform was used.