Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

The Drosophila SAGA complex is composed of the following subunits: Gcn5, Ada2b, dik, Sgf29, CG6506, Ada1-2, Spt3, Spt20, Nipped-A, e(y)1 (Taf9), Taf10b, Taf12, wda, Saf6, not, Sgf11, e(y)2 and Atxn7.

Tandem affinity purification of bait and identification of prey by MudPIT.

Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct in nervous system (elav-GAL4); prey produced from endogenous gene.

All SAGA subunits were present in purifications from both muscle and neuronal cells.

Proteins that non-specifically bind FLAG beads were identified in control purifications from untagged extract (OregonR) and excluded from further analysis.

Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct in muscle (Mef2-GAL4); prey produced from endogenous gene.

All SAGA subunits were present in purifications from both muscle and neuronal cells.

Proteins that non-specifically bind FLAG beads were identified in control purifications from untagged extract (OregonR) and excluded from further analysis.

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Tandem affinity purification of bait.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

The majority of Atxn7 and Ada2b copurify together as part of a 2 MDa complex.

The SAGA complex, as tracked by Ada2b immunoblotting, elutes as a smaller complex in gel filtration in an Atxn7 mutant background, suggesting that SAGA complex members are lost.

Atxn7 complexes were purified and fractionated by gel filtration.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

The Drosophila SAGA complex is composed of the following subunits: Gcn5, Ada2b, dik, Sgf29, CG6506, Ada1-2, Spt3, Spt20, Nipped-A, e(y)1 (Taf9), Taf10b, Taf12, wda, Saf6, not, Sgf11, e(y)2 and Atxn7.

Tandem affinity purification of bait and identification of prey by MudPIT.