Source was embryos of transgenic fly line; proteins produced from endogenous gene.

Characterization of Sas-4 complexes shows that cp309 and Cp190 cosediment in a narrow range of fractions at 7S.

TAP-tagged Sas-4 was immunoprecipitated from "high-speed lysates" depleted of mature centrosomes, and purified complexes were subjected to velocity sedimentation.