Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

E(spl)mγ-HLH interacts with ac, sc, ase, da and gro, but not with E(spl)m3-HLH, in this assay.

Two-hybrid system: yeast GAL4-BD/VP16-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/B42-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast LexA-BD/VP16-AD

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

Mutations in gro that disrupt binding to the WRPW motif typically also disrupt binding of the eh1 motif (FxIxxIL), except for the L692F and L651F mutations, which disrupt only WRPW binding. The V435A and V435L mutations have no effect on gro binding to either the WRPW motif or eh1 domain.

The authors tested the effect of many gro mutations, originally identified as enhancers of h (hairy), for their effects on gro binding to GST bearing a C-terminal WRPW motif. This interaction was inferred by curator to apply not only to the h (hairy) protein, but also to other proteins with a C-terminal WRPW motif: dpn, Sidpn, Him, Hesr and various E(spl) proteins.