FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Fmr1, futsch
FlyBase ID
FBig0000098027
Interaction Network
Interactions Browser links
Fmr1 network
futsch network
Reported Interactions
FBrf0141416-2.coIP.PCR
Reference
Zhang et al., 2001
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Fmr1 

dFXR

bait
futsch 

futsch

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Zhang et al., 2001)

futsch protein level is increased in Fmr1 mutants.

(Zhang et al., 2001)
FBrf0219769-2.coIP.PCR
Reference
Liu et al., 2012
Description
physical association
Assay
Collection
Source/Stage
brain / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Fmr1 

Fmr1

bait
futsch 

futsch

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval brains; bait produced from endogenous gene; prey produced from endogenous gene.

(Liu et al., 2012)
FBrf0221678-3.coIP.PCR
Reference
Cvetkovska et al., 2013
Description
physical association
Assay
Collection
Source/Stage
larval brain / wandering third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Fmr1 

FMRP

bait
futsch 

Futsch

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval brain of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Cvetkovska et al., 2013)

Positive control.

(Cvetkovska et al., 2013)
FBrf0236412-2.coIP.PCR
Reference
Vita and Broadie, 2017
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Fmr1 

dFMRP

bait
futsch 

futsch

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads; bait produced from endogenous gene; prey produced from endogenous gene.

(Vita and Broadie, 2017)
FBrf0242721-2.coIP.PCR
Reference
Sears et al., 2019
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Fmr1 

FMRP

bait
futsch 

futsch

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(Sears et al., 2019)

Source was adult heads; bait produced from endogenous gene; prey produced from endogenous gene.

(Sears et al., 2019)
FBrf0243358-2.coIP.PCR
Reference
Russo and DiAntonio, 2019
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Fmr1 

dFMRP

bait
futsch 

Futsch

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads; bait produced from endogenous gene; prey produced from endogenous gene.

(Russo and DiAntonio, 2019)

Positive control.

(Russo and DiAntonio, 2019)
FBrf0248067-13.coIP.PCR
Reference
Worpenberg et al., 2021
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Fmr1 

Fmr1

bait

GFP tag

futsch 

futsch

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Worpenberg et al., 2021)
FBrf0248067-15.coIP.PCR
Reference
Worpenberg et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Fmr1 

Fmr1

bait

Myc tag

futsch 

futsch

prey
futsch 
futsch 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
futsch 
5-prime UTR
sufficient binding region
futsch 
5-prime UTR methylation sites
mutation disrupting interaction

AGACA to AGTCA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Worpenberg et al., 2021)

Coexpression of Ythdf increases the Fmr1-futsch interaction.

(Worpenberg et al., 2021)
FBrf0253344-1.coIP.PCR
Reference
Song et al., 2022
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Fmr1 

dfmr1

bait

YFP tag

futsch 

Futsch

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult males testes; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Song et al., 2022)

Positive control

(Song et al., 2022)
FBrf0255597-1.coIP.PCR
Reference
Leahy et al., 2023
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Fmr1 

FMRP

bait

YFP tag

futsch 

Futsch

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control

(Leahy et al., 2023)

Source was third instar larvae; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Leahy et al., 2023)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (9)