FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Dronc, Diap1
FlyBase ID
FBig0000098041
Interaction Network
Interactions Browser links
Dronc network
Diap1 network
Reported Interactions
FBrf0174987-2.ENZ.MW
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Dronc 

Dronc

enzyme
Dronc 

Dronc

enzyme
Diap1 

DIAP1

enzyme target
Diap1 
Diap1 

DIAP1

enzyme target
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0192294-2.coIP.WB
Reference
Means et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Dronc 

DRONC

bait

His tag

Diap1 

DIAP1

prey

GST tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

An N-terminal hid peptide lacking the initiator methionine can disrupt the interaction between Diap1 and active Dronc but not the catalytically inactive Dronc C318S.

(Means et al., 2006)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Means et al., 2006)
FBrf0195898-1.ENZ.WB
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Dronc 

DRONC

enzyme

HIS tag

Dronc 

DRONC

enzyme

HIS tag

Diap1 

Diap1

enzyme target

GST tag

Diap1 

Diap1

enzyme target

GST tag

Diap1 
Diap1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0195898-2.PD.A
Reference
Muro et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Dronc 

DRONC

bait

GST tag

Diap1 

Diap1

prey
Diap1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Diap1 
Spacer Region
mutation decreasing interaction
aa 205

E205A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Muro et al., 2005)
FBrf0204202-1.coIP.WB
Reference
Dorstyn and Kumar, 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
ML-DmBG2
Participants
Corresponds to
Reported as
Role
Note
Dronc 

DRONC

bait

His tag

Diap1 

DIAP1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The Dronc-Diap1 interaction is not detected when cells are treated with cyclohexamide.

(Dorstyn and Kumar, 2008)

Source was cell extract of BG2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Dorstyn and Kumar, 2008)
FBrf0206305-1.coIP.WB
Reference
Ditzel et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Dronc 

DRONC

bait

V5 tag

Diap1 

DIAP1

prey
Diap1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Diap1 
N-terminal domain
mutation increasing interaction
aa 1-20

deletion of N-terminus

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of 293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ditzel et al., 2008)
FBrf0213078-1.coIP.WB
Reference
Rumpf et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Diap1 

DIAP1

bait

HA tag

Dronc 

Dronc

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(Rumpf et al., 2011)

The interaction of th with Nc is not disrupted by coexpression of TER94.

(Rumpf et al., 2011)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Rumpf et al., 2011)
FBrf0226660-3.coIP.WB
Reference
Huang et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Dronc 

Dronc

bait

Myc tag

Diap1 

DIAP1

prey

V5 tag

Diap1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Diap1 
ΔN form
sufficient binding region
aa 21-438

mimics the caspase cleaved form

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(Huang et al., 2014)

The Dronc-Diap1 interaction is stronger when Ubr3 is co-expressed.

(Huang et al., 2014)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Huang et al., 2014)
FBrf0259668-4.coIP.WB
Reference
Liu et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Diap1 

Diap1

bait

FLAG tag

Dronc 

Dronc

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Liu et al., 2021)
FBrf0259668-5.coIP.WB
Reference
Liu et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Dronc 

Dronc

bait

Myc tag

Diap1 

Diap1

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Addition of rdx reduces the interaction between Dronc and Diap1

(Liu et al., 2021)

Source was cell extract of 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Liu et al., 2021)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (8)