Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Gradient sedimentation of S2 cells indicates that Arfip is also present in 17S fractions known to contain dynactin complexes.

Source was sedimentation fraction of adult head lysate of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

Adult head lysate subjected to sucrose gradient sedimentation, and 17S fractions known to contain large dynactin complexes were used for coimmunoprecipitation analysis.