pA2-I binding site, diagrammed in Fig1E.
Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.
genomic coordinates inferred from Fig2B
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.
genomic coordinates inferred from Fig2B
Source was embryos of transgenic fly line; bait produced from endogenous gene; prey produced from transgenic construct.
A shorter sufficient binding region as well as mutations that disrupt binding for ewg are shown but details for these constructs are unclear.
RNA immunoprecipitated from formaldehyde fixed embryos.
elav protein binds transcripts with developmentally regulated 3\' UTR extensions.
Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced as a recombinant fusion protein in bacterial system.
Radiolabeled RNA and purified protein were UV cross-linked, treated with RNaseA and protected, labeled RNA was visualized by gel electrophoresis and autoradiography.
Positive control.
Positive control.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.
